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Eighty-one strains of Neisseria gonorrhoeae were isolated, identified from 320 clinical specimens and further characterized on the effects of VCN and isovitalex, on the utilization of carbon sources, on the production of fl-lactamase, and on plasmid patterns. Out of the 81, seventy-two strains were identified as N. gonorrhoeae on chocolate agar, 80 on Thayer-Martin medium, and 55 on 2% isovitalex Thayer-Martin medium. Out of the 81, sixty-seven strains produced acid at 48-hour culture in glucose medium, and 10 did it at 72 hours, but 4 did not produce it at 72 hours. Fourty-one strains out of the 81 produced R-lactamase, in which one strain (PL-118) contained 2.6,4.5 and 24.5 Mdaltons plasmids.
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